Search results for "Lambda phage"

showing 3 items of 3 documents

A new assay for O6-alkylguanine-DNA-alkyltransferase to determine DNA repair capacities using lambda-phage DNA as substrate.

1990

One O6-methylguanine (O6-meG) was introduced into each BamHI site of lambda-phage DNA as a substrate for the determination of the DNA repair protein O6-alkylguanine-DNA-alkyltransferase. A new assay using as the detection group 32P-labeled phosphate introduced at the 3' position of the modified nucleoside by incorporation of 32P-labeled TTP in the 3'-neighboring position proved highly sensitive: 10(-16) mol of the DNA lesion was still easily detectable. This DNA, which has greater than 1000 bp represents a good model for cellular DNA and was used as a substrate to measure the individual repair capacities for O6-meG in human lymphocytes of 20 healthy male and female donors. There were great …

Cancer ResearchGuanineDNA RepairDNA repairMolecular Sequence DataBiologySubstrate Specificitychemistry.chemical_compoundO(6)-Methylguanine-DNA MethyltransferaseDNA Repair ProteinEscherichia coliHumansLymphocyteschemistry.chemical_classificationBase SequenceSubstrate (chemistry)General MedicineMethyltransferasesLambda phagebiology.organism_classificationBacteriophage lambdaIn vitroKineticsEnzymeBiochemistrychemistryDNA ViralBamHIDNACarcinogenesis
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The biological activity of bacteriophage DNA, prepared by the cationic detergent dilution technique

1975

Abstract The preparation of phage lambda DNA infecting E. coli K 12 with cationic detergent is described. This DNA infects E. coli spheroblasts with the same efficiency as DNA prepared by phenol methods.

DetergentsViral Plaque AssayBiologyVirus Replicationmedicine.disease_causeColiphagesBacteriophagechemistry.chemical_compoundEscherichia coliGeneticsmedicinePhenolEscherichia coliVirus quantificationDNA VirusesBiological activityLambda phageChromatography Ion Exchangebiology.organism_classificationMolecular biologyQuaternary Ammonium CompoundschemistryBiochemistryViral replicationDNA ViralDNANucleic Acids Research
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Restriction analysis of lambda EMBL3 background recombinants: occurrence of lambda phages carrying a head to tail oriented left arm DNA sequence.

1989

Eight representative recombinant background clones of lambda EMBL3 were analysed using KpnI, BamHI, SalI, EcoRI and HindIII digestion. We found that lambda EMBL3 carries its own left arm in the BamHI cloning site. In the way, recombinant molecules were found to be generated which can grow on Escherichia coli strain NM539. In all cases analysed, the left arm DNA was inserted in a head to tail orientation. Seven clones carried a restored BamHI site at the cos site-BamHI site connection. In the region where the inserted left arm and the right arm were ligated, BamHI cloning produces a large palindromic sequence consisting of two polylinkers. This BamHI site was incompletely cleaved in all case…

GeneticsbiologyDeoxyribonuclease BamHIGenetic VectorsEcoRINucleic acid sequenceChromosome MappingLambda phageMolecular cloningbiology.organism_classificationMolecular biologyBacteriophage lambdalaw.inventionlawCloning SiteDNA ViralGeneticsbiology.proteinRecombinant DNAEscherichia coliNucleic Acid ConformationBamHICloning MolecularMolecular BiologyPalindromic sequenceMoleculargeneral genetics : MGG
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